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Image Search Results
Journal: Translational Research in Anatomy
Article Title: JAK-1/STAT-3 pathway mediated role in aging cerebellar cortex degenerative changes of albino wistar rats
doi: 10.1016/j.tria.2020.100089
Figure Lengend Snippet: Fig. 2. (A–C) Photomicrographs of rats' cerebellar cortex stained with anti-GFAP antibodies (X 1000). (A) Represents 12M-group, with mild GFAP immunoreaction while (B) and (C) represents 24M (moderate reaction) and 32M (sever reaction) groups respectively. Note: GFAP positive cells (arrow) appeared with dark brown discoloration. (D) Represents GFAP immunoreaction changes in different age groups. * statistically significant (p < 0.05) difference in comparison to 12M-group. ** statistically significant (p < 0.05) difference in comparison to 24M-group. Data are presented as mean ± standard deviation, (n = 30). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Article Snippet:
Techniques: Staining, Comparison, Standard Deviation
Journal: Medicine
Article Title: Aberrant PTEN, PIK3CA, pMAPK, and TP53 expression in human scalp and face angiosarcoma
doi: 10.1097/md.0000000000026779
Figure Lengend Snippet: Figure 3. (A) PIK3CA immunoreactivity in angiosarcoma of the scalp and face and hemangioma. Angiosarcoma lesions exhibited significantly higher PIK3CA immunoreactivity than hemangioma (x2=20.97, P=.001, P<.01). (B) Representative images of immunohistochemical staining of PIK3CA in (a) angiosarcoma and (b) hemangioma tissues (SP, 100). PIK3CA=phospha- tidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha.
Article Snippet: The slides were incubated overnight at 4°C with a rabbit polyclonal antibody against human PTEN,
Techniques: Immunohistochemical staining, Staining
Journal: Journal of Neuroinflammation
Article Title: Inhibition of HMGB1 reduces rat spinal cord astrocytic swelling and AQP4 expression after oxygen-glucose deprivation and reoxygenation via TLR4 and NF-κB signaling in an IL-6-dependent manner
doi: 10.1186/s12974-017-1008-1
Figure Lengend Snippet: Spinal cord astrocyte identification and high mobility group box-1 (HMGB1) knockdown. a Spinal cord astrocytes were identified using immunofluorescence. The percentage of cells stained with the astrocytic marker S100β, which were identified as astrocytes, was more than 95% of the total cells (three replicates). b HMGB1 knockdown efficiency in the plasma membrane and cytoplasm of spinal cord astrocytes was evaluated using Western blot for HMGB1 protein levels. Results were obtained after 72 h of specific HMGB1 shRNA treatment. HMGB1 protein levels were decreased to approximately 30% of normal levels with shRNA multiplicity of infection 60 as compared to normal astrocytes. * P < 0.05 vs. normal group (three replicates)
Article Snippet: Primary antibodies include rabbit polyclonal anti-HMGB1 antibody for rat, mouse, and human (Abcam, Cat# ab18256, RRID:AB_444360, Cambridge, UK); rabbit polyclonal anti-AQP4 antibody for rat, mouse, human, and pig (Abcam, Cat# ab46182, RRID: AB_955676); mouse monoclonal anti-TLR4 antibody for rat, mouse, human, pig, baboon, bovine, and Chinese hamster (Novus, Cat# 76B357.1, RRID: AB_839000, Littleton, CO, USA); rabbit polyclonal anti-TLR4 antibody for rat, mouse, human, and rabbit (Boster, Cat# BA1717, RRID:AB_2716293); rabbit polyclonal anti-myeloid differentiation primary response gene 88 (MyD88) antibody for rat and human (Abcam, Cat# ab131071, RRID: AB_11156885); mouse monoclonal anti-IκBα antibody for rat, mouse, human, monkey, bovine, pig, and guinea pig (Cell Signaling Technology, Cat# 4814, RRID: AB_390781, Boston, MA, USA); mouse monoclonal anti-p-IκBα antibody for rat, mouse, human, and monkey (Cell Signaling Technology, Cat# 9246, RRID:AB_2267145); rabbit monoclonal anti-NF-κB antibody for rat, mouse, human, monkey, and bovine (Cell Signaling Technology, Cat# 4764, RRID:AB_823578); mouse monoclonal anti-GAPDH antibody for rat, mouse, and human (Beyotime, Cat# AF0006, RRID: AB_2715590, Shanghai, China); mouse monoclonal anti-Histone H3 antibody for rat, mouse, and human (Beyotime, Cat# AF0009, RRID: AB_2715593); and
Techniques: Knockdown, Immunofluorescence, Staining, Marker, Clinical Proteomics, Membrane, Western Blot, shRNA, Infection